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TARGET-Seq: A Protocol for High-Sensitivity Single-Cell Mutational Analysis and Parallel RNA Sequencing

Single-cell RNA-sequencing technologies are ideally placed to resolve intratumoral heterogeneity. However, the lack of coverage across key mutation hotspots has precluded the correlation of genetic and transcriptional readouts from the same single cell. To overcome this, we developed TARGET-seq, a p...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Rodriguez-Meira, Alba, O’Sullivan, Jennifer, Rahman, Haseeb, Mead, Adam J.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757016/
https://ncbi.nlm.nih.gov/pubmed/33377019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100125
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