RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters

Biosynthesis reprograming is an important way to diversify chemical structures. The large repetitive DNA sequences existing in polyketide synthase genes make seamless DNA manipulation of the polyketide biosynthetic gene clusters extremely challenging. In this study, to replace the ethyl group attach...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Song, Chaoyi, Luan, Ji, Li, Ruijuan, Jiang, Chanjuan, Hou, Yu, Cui, Qingwen, Cui, Tianqi, Tan, Long, Ma, Zaichao, Tang, Ya-Jie, Stewart, A Francis, Fu, Jun, Zhang, Youming, Wang, Hailong
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2020
Fag:
Methods Online
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7736807/
https://ncbi.nlm.nih.gov/pubmed/33119745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa956
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