RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters

Biosynthesis reprograming is an important way to diversify chemical structures. The large repetitive DNA sequences existing in polyketide synthase genes make seamless DNA manipulation of the polyketide biosynthetic gene clusters extremely challenging. In this study, to replace the ethyl group attach...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Song, Chaoyi, Luan, Ji, Li, Ruijuan, Jiang, Chanjuan, Hou, Yu, Cui, Qingwen, Cui, Tianqi, Tan, Long, Ma, Zaichao, Tang, Ya-Jie, Stewart, A Francis, Fu, Jun, Zhang, Youming, Wang, Hailong
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7736807/
https://ncbi.nlm.nih.gov/pubmed/33119745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa956
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