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Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)
Directed evolution methodologies benefit from read-outs quantitatively linking genotype to phenotype. We therefore devised a method that couples protein–peptide interactions to the dynamic read-out provided by an engineered DNA polymerase. Fusion of a processivity clamp protein to a thermostable nuc...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2020
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7736784/ https://ncbi.nlm.nih.gov/pubmed/33104786 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa933 |
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