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Directed co-evolution of interacting protein–peptide pairs by compartmentalized two-hybrid replication (C2HR)
Directed evolution methodologies benefit from read-outs quantitatively linking genotype to phenotype. We therefore devised a method that couples protein–peptide interactions to the dynamic read-out provided by an engineered DNA polymerase. Fusion of a processivity clamp protein to a thermostable nuc...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7736784/ https://ncbi.nlm.nih.gov/pubmed/33104786 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa933 |
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