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Directed evolution of polymerase function by compartmentalized self-replication
We describe compartmentalized self-replication (CSR), a strategy for the directed evolution of enzymes, especially polymerases. CSR is based on a simple feedback loop consisting of a polymerase that replicates only its own encoding gene. Compartmentalization serves to isolate individual self-replica...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
2001
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC31872/ https://ncbi.nlm.nih.gov/pubmed/11274352 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.071052198 |
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