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Compartmentalized partnered replication for the directed evolution of genetic parts and circuits

Compartmentalized partnered replication (CPR) is an emulsion-based directed evolution method based on a robust and modular phenotype-genotype linkage. In contrast to other in vivo directed evolution approaches, CPR largely mitigates host fitness effects due to a relatively short expression time of t...

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Podrobná bibliografie
Vydáno v:Nat Protoc
Hlavní autoři: Abil, Zhanar, Ellefson, Jared W, Gollihar, Jimmy D, Watkins, Ella, Ellington, Andrew D
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6053311/
https://ncbi.nlm.nih.gov/pubmed/29120463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.119
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