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Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization
We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Copyright 2005 by RNA Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370839/ https://ncbi.nlm.nih.gov/pubmed/16131588 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2121705 |
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