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Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization

We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically...

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Hlavní autoři: LEVY, MATTHEW, GRISWOLD, KARL E., ELLINGTON, ANDREW D.
Médium: Artigo
Jazyk:Inglês
Vydáno: Copyright 2005 by RNA Society 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370839/
https://ncbi.nlm.nih.gov/pubmed/16131588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2121705
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