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Selection of ribozymes that catalyse multiple-turnover Diels–Alder cycloadditions by using in vitro compartmentalization

In vitro compartmentalization (IVC) has previously been used to evolve protein enzymes. Here, we demonstrate how IVC can be applied to select RNA enzymes (ribozymes) for a property that has previously been unselectable: true intermolecular catalysis. Libraries containing 10(11) ribozyme genes are co...

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Detalhes bibliográficos
Main Authors: Agresti, Jeremy J., Kelly, Bernard T., Jäschke, Andres, Griffiths, Andrew D.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1283419/
https://ncbi.nlm.nih.gov/pubmed/16260754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0503733102
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