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Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase

The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA poly...

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Bibliografische gegevens
Hoofdauteurs: Xia, Gang, Chen, Liangjing, Sera, Takashi, Fa, Ming, Schultz, Peter G., Romesberg, Floyd E.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The National Academy of Sciences 2002
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC124448/
https://ncbi.nlm.nih.gov/pubmed/12011423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.102577799
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