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Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase
The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA poly...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC124448/ https://ncbi.nlm.nih.gov/pubmed/12011423 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.102577799 |
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