Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase
The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA poly...
Zapisane w:
| Wydane w: | Proc Natl Acad Sci U S A |
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| Główni autorzy: | , , , , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
National Academy of Sciences
2002
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC124448/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12011423/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.102577799 |
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