Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase

The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA poly...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής

Εκδόθηκε σε:	Proc Natl Acad Sci U S A
Κύριοι συγγραφείς:	Xia, Gang, Chen, Liangjing, Sera, Takashi, Fa, Ming, Schultz, Peter G., Romesberg, Floyd E.
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	National Academy of Sciences 2002
Θέματα:	Biological Sciences
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC124448/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12011423/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.102577799
Ετικέτες:	Προσθήκη ετικέτας Δεν υπάρχουν, Καταχωρήστε ετικέτα πρώτοι!