Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase

The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA poly...

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Publicat a:	Proc Natl Acad Sci U S A
Autors principals:	Xia, Gang, Chen, Liangjing, Sera, Takashi, Fa, Ming, Schultz, Peter G., Romesberg, Floyd E.
Format:	Artigo
Idioma:	Inglês
Publicat:	National Academy of Sciences 2002
Matèries:	Biological Sciences
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC124448/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12011423/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.102577799
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