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Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing. Additionally, no study has reported the possibili...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Tanida, Isei, Furuta, Yoko, Yamaguchi, Junji, Kakuta, Soichiro, Oliva Trejo, Juan Alejandro, Uchiyama, Yasuo
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7736269/
https://ncbi.nlm.nih.gov/pubmed/33318540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-78879-x
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