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Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein

Post-fixation with osmium tetroxide staining and the embedding of Epon are robust and essential treatments that are used to preserve and visualize intracellular membranous structures during electron microscopic analyses. These treatments, however, can significantly diminish the fluorescent intensity...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Tanida, Isei, Kakuta, Soichiro, Oliva Trejo, Juan Alejandro, Uchiyama, Yasuo
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7347593/
https://ncbi.nlm.nih.gov/pubmed/32647231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-68191-z
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