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Characterization of starvation-induced autophagy in cerebellar Purkinje cells of pHluorin-mKate2-human LC3B transgenic mice

We generated a new transgenic mouse model that expresses a pHluorin-mKate2 fluorescent protein fused with human LC3B (PK-LC3 mice) for monitoring autophagy activity in neurons of the central nervous system. Histological analysis revealed fluorescent puncta in neurons of the cerebral cortex, hippocam...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Oliva Trejo, Juan Alejandro, Tanida, Isei, Suzuki, Chigure, Kakuta, Soichiro, Tada, Norihiro, Uchiyama, Yasuo
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7295967/
https://ncbi.nlm.nih.gov/pubmed/32541814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-66370-6
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