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Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins

In-resin CLEM of Epon embedded samples can greatly simplify the correlation of fluorescent images with electron micrographs. The usefulness of this technique is limited at present by the low number of fluorescent proteins that resist CLEM processing. Additionally, no study has reported the possibili...

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Publicado en:Sci Rep
Autores principales: Tanida, Isei, Furuta, Yoko, Yamaguchi, Junji, Kakuta, Soichiro, Oliva Trejo, Juan Alejandro, Uchiyama, Yasuo
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group UK 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7736269/
https://ncbi.nlm.nih.gov/pubmed/33318540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-78879-x
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