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Kinetic analysis of the intracellular processing of siRNAs by confocal microscopy

Here, we describe a method for tracking intracellular processing of small interfering RNA (siRNA) containing complexes using automated microscopy controls and image acquisition to minimize user effort and time. This technique uses fluorescence colocalization to monitor dual-labeled fluorescent siRNA...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Microscopy (Oxf)
Main Authors: Vocelle, Daniel, Chesniak, Olivia M, Smith, Milton R, Chan, Christina, Walton, S Patrick
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7711900/
https://ncbi.nlm.nih.gov/pubmed/32525202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jmicro/dfaa031
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