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Kinetic analysis of the intracellular processing of siRNAs by confocal microscopy

Here, we describe a method for tracking intracellular processing of small interfering RNA (siRNA) containing complexes using automated microscopy controls and image acquisition to minimize user effort and time. This technique uses fluorescence colocalization to monitor dual-labeled fluorescent siRNA...

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Detalles Bibliográficos
Publicado en:Microscopy (Oxf)
Main Authors: Vocelle, Daniel, Chesniak, Olivia M, Smith, Milton R, Chan, Christina, Walton, S Patrick
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7711900/
https://ncbi.nlm.nih.gov/pubmed/32525202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jmicro/dfaa031
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