Kinetic analysis of the intracellular processing of siRNAs by confocal microscopy

Here, we describe a method for tracking intracellular processing of small interfering RNA (siRNA) containing complexes using automated microscopy controls and image acquisition to minimize user effort and time. This technique uses fluorescence colocalization to monitor dual-labeled fluorescent siRNA...

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Bibliografiske detaljer
Udgivet i:Microscopy (Oxf)
Main Authors: Vocelle, Daniel, Chesniak, Olivia M, Smith, Milton R, Chan, Christina, Walton, S Patrick
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2020
Fag:
Technical Report
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7711900/
https://ncbi.nlm.nih.gov/pubmed/32525202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jmicro/dfaa031
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