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A robust CRISPR–Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair

DNA double-strand breaks (DSBs) are highly cytotoxic lesions that can lead to chromosome rearrangements, genomic instability and cell death. Consequently, cells have evolved multiple mechanisms to efficiently repair DSBs to preserve genomic integrity. We have developed a DSB repair assay system, des...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Eki, Rebeka, She, Jane, Parlak, Mahmut, Benamar, Mouadh, Du, Kang-Ping, Kumar, Pankaj, Abbas, Tarek
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708081/
https://ncbi.nlm.nih.gov/pubmed/33068408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa897
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