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A robust CRISPR–Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair

DNA double-strand breaks (DSBs) are highly cytotoxic lesions that can lead to chromosome rearrangements, genomic instability and cell death. Consequently, cells have evolved multiple mechanisms to efficiently repair DSBs to preserve genomic integrity. We have developed a DSB repair assay system, des...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Eki, Rebeka, She, Jane, Parlak, Mahmut, Benamar, Mouadh, Du, Kang-Ping, Kumar, Pankaj, Abbas, Tarek
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708081/
https://ncbi.nlm.nih.gov/pubmed/33068408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa897
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