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A robust CRISPR–Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair

DNA double-strand breaks (DSBs) are highly cytotoxic lesions that can lead to chromosome rearrangements, genomic instability and cell death. Consequently, cells have evolved multiple mechanisms to efficiently repair DSBs to preserve genomic integrity. We have developed a DSB repair assay system, des...

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Eki, Rebeka, She, Jane, Parlak, Mahmut, Benamar, Mouadh, Du, Kang-Ping, Kumar, Pankaj, Abbas, Tarek
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708081/
https://ncbi.nlm.nih.gov/pubmed/33068408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa897
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