Marker-free quantification of repair pathway utilization at Cas9-induced double-strand breaks

Genome integrity and genome engineering require efficient repair of DNA double-strand breaks (DSBs) by non-homologous end joining (NHEJ), homologous recombination (HR), or alternative end-joining pathways. Here we describe two complementary methods for marker-free quantification of DSB repair pathwa...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Feng, Wanjuan, Simpson, Dennis A, Cho, Jang-Eun, Carvajal-Garcia, Juan, Smith, Chelsea M, Headley, Kathryn M, Hathaway, Nate, Ramsden, Dale A, Gupta, Gaorav P
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2021
Assuntos:
Genome Integrity, Repair and Replication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8136827/
https://ncbi.nlm.nih.gov/pubmed/33963863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkab299
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