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Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP

Multidrug transporters can confer drug resistance on cells by extruding structurally unrelated compounds from the cellular interior. In transport assays, Hoechst 33342 (referred to as Hoechst) is a commonly used substrate, the fluorescence of which changes in the transport process. With three basic...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Swain, Brendan M., Guo, Dawei, Singh, Himansha, Rawlins, Philip B., McAlister, Mark, van Veen, Hendrik W.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7674423/
https://ncbi.nlm.nih.gov/pubmed/33208856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-76943-0
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