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Complexities of a protonatable substrate in measurements of Hoechst 33342 transport by multidrug transporter LmrP

Multidrug transporters can confer drug resistance on cells by extruding structurally unrelated compounds from the cellular interior. In transport assays, Hoechst 33342 (referred to as Hoechst) is a commonly used substrate, the fluorescence of which changes in the transport process. With three basic...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Swain, Brendan M., Guo, Dawei, Singh, Himansha, Rawlins, Philip B., McAlister, Mark, van Veen, Hendrik W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7674423/
https://ncbi.nlm.nih.gov/pubmed/33208856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-76943-0
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