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Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility

CRISPR-Cas9 genome engineering is a powerful technology for correcting genetic diseases. However, the targeting range of Cas9 proteins is limited by their requirement for a protospacer adjacent motif (PAM) and in vivo delivery is challenging due to their large size. Here, we use phage-assisted conti...

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שמור ב:
מידע ביבליוגרפי
הוצא לאור ב:Nat Chem Biol
Main Authors: Schmidheini, Lukas, Mathis, Nicolas, Marquart, Kim Fabiano, Rothgangl, Tanja, Kissling, Lucas, Böck, Desirée, Chanez, Christelle, Wang, Jingrui Priscilla, Jinek, Martin, Schwank, Gerald
פורמט: Artigo
שפה:Inglês
יצא לאור: 2024
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC7616171/
https://ncbi.nlm.nih.gov/pubmed/37735239
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41589-023-01427-x
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