Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids

This protocol describes the isolation, handling, culture of, and experiments with human colon stem cell organoids in the context of cystic fibrosis (CF). In human colon organoids, the function of cystic fibrosis transmembrane conductance regulator (CFTR) protein and its rescue by CFTR modulators can...

詳細記述

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書誌詳細
出版年:STAR Protoc
主要な著者: Vonk, Annelotte M., van Mourik, Peter, Ramalho, Anabela S., Silva, Iris A.L., Statia, Marvin, Kruisselbrink, Evelien, Suen, Sylvia W.F., Dekkers, Johanna F., Vleggaar, Frank P., Houwen, Roderick H.J., Mullenders, Jasper, Boj, Sylvia F., Vries, Robert, Amaral, Margarida D., de Boeck, Kris, van der Ent, Cornelis K., Beekman, Jeffrey M.
フォーマット: Artigo
言語:Inglês
出版事項: Elsevier 2020
主題:
Protocol
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580120/
https://ncbi.nlm.nih.gov/pubmed/33111074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100019
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