Protocol for Application, Standardization and Validation of the Forskolin-Induced Swelling Assay in Cystic Fibrosis Human Colon Organoids

This protocol describes the isolation, handling, culture of, and experiments with human colon stem cell organoids in the context of cystic fibrosis (CF). In human colon organoids, the function of cystic fibrosis transmembrane conductance regulator (CFTR) protein and its rescue by CFTR modulators can...

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Vydáno v:STAR Protoc
Hlavní autoři: Vonk, Annelotte M., van Mourik, Peter, Ramalho, Anabela S., Silva, Iris A.L., Statia, Marvin, Kruisselbrink, Evelien, Suen, Sylvia W.F., Dekkers, Johanna F., Vleggaar, Frank P., Houwen, Roderick H.J., Mullenders, Jasper, Boj, Sylvia F., Vries, Robert, Amaral, Margarida D., de Boeck, Kris, van der Ent, Cornelis K., Beekman, Jeffrey M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2020
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580120/
https://ncbi.nlm.nih.gov/pubmed/33111074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100019
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