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Reduced Expression of VEGF-A in Human Retinal Pigment Epithelial Cells and Human Muller Cells Following CRISPR-Cas9 Ribonucleoprotein-Mediated Gene Disruption

PURPOSE: To evaluate the effects of vascular endothelial growth factor-A (VEGF-A) gene editing in human retinal pigment epithelial (RPE) cells and human Muller cells, which are the main VEGF-A producing cells in the eye. METHODS: CRISPR-Cas9 ribonucleoprotein was used to target exon 1 in VEGF-A gene...

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Vydáno v:Transl Vis Sci Technol
Hlavní autoři: Ameri, Hossein, Murat, Christopher, Arbabi, Amirmohsen, Jiang, Wei, Janga, Srikanth R., Qin, Peter Zhifeng, Hamm-Alvarez, Sarah F.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Association for Research in Vision and Ophthalmology 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7422915/
https://ncbi.nlm.nih.gov/pubmed/32855870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1167/tvst.9.8.23
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