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Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins
Genome editing in human cells with targeted nucleases now enables diverse experimental and therapeutic genome engineering applications, but extension to primary human B cells remains limited. Here we report a method for targeted genetic engineering in primary human B cells, utilizing electroporation...
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| 發表在: | J Immunol Methods |
|---|---|
| Main Authors: | , , , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2018
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6124898/ https://ncbi.nlm.nih.gov/pubmed/29614266 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2018.03.009 |
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