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CRISPR-Cas9 genome engineering of primary CD4(+) T cells for the interrogation of HIV-host factor interactions

CRISPR-Cas9 gene-editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological processes. We have recently adapted this technology for use in primary human CD4(+) T cells to create a high-throughput platform for analyzing th...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Hultquist, Judd F., Hiatt, Joseph, Schumann, Kathrin, McGregor, Michael J., Roth, Theodore L., Haas, Paige, Doudna, Jennifer A., Marson, Alexander, Krogan, Nevan J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6637941/
https://ncbi.nlm.nih.gov/pubmed/30559373
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0069-7
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