A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells

New genetic tools are needed to understand the functional interactions between HIV and human host factors in primary cells. We recently developed a method to edit the genome of primary CD4+ T cells by electroporation of CRISPR/Cas9 ribonucleoproteins (RNPs). Here, we adapted this methodology to a hi...

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Detalhes bibliográficos
Publicado no:Cell Rep
Main Authors: Hultquist, Judd F., Schumann, Kathrin, Woo, Jonathan M., Manganaro, Lara, McGregor, Michael J., Doudna, Jennifer, Simon, Viviana, Krogan, Nevan J., Marson, Alexander
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5123761/
https://ncbi.nlm.nih.gov/pubmed/27783955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2016.09.080
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