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Competition NMR for Detection of Hit/Lead Inhibitors of Protein–Protein Interactions

Screening for small-molecule fragments that can lead to potent inhibitors of protein–protein interactions (PPIs) is often a laborious step as the fragments cannot dissociate the targeted PPI due to their low μM–mM affinities. Here, we describe an NMR competition assay called w-AIDA-NMR (weak-antagon...

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Veröffentlicht in:Molecules
Hauptverfasser: Musielak, Bogdan, Janczyk, Weronika, Rodriguez, Ismael, Plewka, Jacek, Sala, Dominik, Magiera-Mularz, Katarzyna, Holak, Tad
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7412237/
https://ncbi.nlm.nih.gov/pubmed/32630327
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules25133017
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