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Confluence of Theory and Experiment Reveal the Catalytic Mechanism of the Varkud Satellite Ribozyme

The Varkud Satellite (VS) ribozyme catalyzes site-specific RNA cleavage and ligation, and serves as an important model system to understand RNA catalysis. Here we combine stereospecific phosphorothioate substitution, precision nucleobase mutation and linear free energy relationship measurements with...

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Detalhes bibliográficos
Publicado no:Nat Chem
Main Authors: Ganguly, Abir, Weissman, Benjamin P., Giese, Timothy J., Li, Nan-Sheng, Hoshika, Shuichi, Rao, Saieesh, Benner, Steven A., Piccirilli, Joseph A., York, Darrin M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7389185/
https://ncbi.nlm.nih.gov/pubmed/31959957
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41557-019-0391-x
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