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Deuterium Kinetic Isotope Effects Resolve Low-Temperature Substrate Radical Reaction Pathways and Steps in B(12)-Dependent Ethanolamine Ammonia-Lyase
The first-order reaction kinetics of the cryotrapped 1, 1, 2, 2-(2)H(4)-aminoethanol substrate radical intermediate state in the adenosylcobalamin (B(12)) -dependent ethanolamine ammonia-lyase (EAL) from Salmonella enterica serovar Typhimurium are measured over 203 – 225 K by using time-resolved, fu...
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| Published in: | Biochemistry |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7387329/ https://ncbi.nlm.nih.gov/pubmed/31419122 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.9b00588 |
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