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Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides

Conditional knockout (cKO) mice are extremely valuable for biomedical research because they enable detailed analyses of gene functions in a tissue- or temporally-specific fashion. The conventional method for generating cKO mice is time consuming and labor intensive, which involves making a large gen...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Liu, Ye, Du, Yubin, Xie, Wen, Zhang, Fan, Forrest, Douglas, Liu, Chengyu
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7354058/
https://ncbi.nlm.nih.gov/pubmed/30353515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8831-0_11
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