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Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice

NOD.Cg-Prkdc(scid) Il2rg(tm1Wjl/)SzJ (NSG) mice are an immunodeficient strain that enables human cell xenografts. However, NSG mice possess a complex genetic background that would complicate cross-breeding with other inbred transgenic or knockout mouse strains to establish a congenic strain with a d...

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Publicado en:Methods Mol Biol
Autores principales: Du, Yubin, Xie, Wen, Zhang, Fan, Choi, Uimook, Liu, Chengyu, Sweeney, Colin L
Formato: Artigo
Lenguaje:Inglês
Publicado: 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7467215/
https://ncbi.nlm.nih.gov/pubmed/30353512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8831-0_8
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