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Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids
Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using...
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| Publicado no: | Chem Commun (Camb) |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7337974/ https://ncbi.nlm.nih.gov/pubmed/32462144 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0cc01737j |
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