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Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents

Functional studies of membrane-bound channels, transporters or signal transducers require that the protein of interest resides in a membrane that separates two compartments. One approach that is commonly used to prepare these systems is to reconstitute the protein in liposomes. An intermediate step...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Smirnova, Irina A., Ädelroth, Pia, Brzezinski, Peter
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6175888/
https://ncbi.nlm.nih.gov/pubmed/30297885
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-33208-1
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