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Protein-ligand affinity determinations using covalent labeling-mass spectrometry

Determining binding affinity is an important aspect of charactering protein-ligand complexes. Here, we describe an approach based on covalent labeling (CL)-mass spectrometry (MS) that can accurately provide protein-ligand dissociation constants (K(d) values), using diethylpyrocarbonate (DEPC) as the...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:J Am Soc Mass Spectrom
Prif Awduron: Liu, Tianying, Marcinko, Tyler M., Vachet, Richard W.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7332385/
https://ncbi.nlm.nih.gov/pubmed/32501685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jasms.0c00131
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