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Protein-ligand affinity determinations using covalent labeling-mass spectrometry
Determining binding affinity is an important aspect of charactering protein-ligand complexes. Here, we describe an approach based on covalent labeling (CL)-mass spectrometry (MS) that can accurately provide protein-ligand dissociation constants (K(d) values), using diethylpyrocarbonate (DEPC) as the...
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| Publicado no: | J Am Soc Mass Spectrom |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7332385/ https://ncbi.nlm.nih.gov/pubmed/32501685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jasms.0c00131 |
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