Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion

An orthogonal aminoacyl-tRNA synthetase/tRNA pair is a crucial prerequisite for site-specific incorporation of unnatural amino acids. Due to its high codon suppression efficiency and full orthogonality, the pyrrolysyl-tRNA synthetase/pyrrolysyl-tRNA pair is currently the ideal system for genetic cod...

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Udgivet i:Nat Commun
Main Authors: Ding, Wenlong, Zhao, Hongxia, Chen, Yulin, Zhang, Bin, Yang, Yang, Zang, Jia, Wu, Jing, Lin, Shixian
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7308279/
https://ncbi.nlm.nih.gov/pubmed/32572025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-16898-y
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