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Reaction Mechanism of Glutamate Carboxypeptidase II Revealed by Mutagenesis, X-ray Crystallography, and Computational Methods

Glutamate carboxypeptidase II (GCPII, EC 3.4.17.21) is a zinc-dependent exopeptidase and an important therapeutic target for neurodegeneration and prostate cancer. The hydrolysis of N-acetyl-l-aspartyl-l-glutamate (N-Ac-Asp-Glu), the natural dipeptidic substrate of the GCPII, is intimately involved...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Klusák, Vojtêch, Bařinka, Cyril, Plechanovová, Anna, Mlčochová, Petra, Konvalinka, Jan, Rulíšek, Lubomír, Lubkowski, Jacek
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7289149/
https://ncbi.nlm.nih.gov/pubmed/19301871
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900220s
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