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Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays

We report a tool, Calling Cards Reporter Arrays (CCRA), that measures transcription factor (TF) binding and the consequences on gene expression for hundreds of synthetic promoters in yeast. Using Cbf1p and MAX, we demonstrate that the CCRA method is able to detect small changes in binding free energ...

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Pubblicato in:Nucleic Acids Res
Autori principali: Liu, Jiayue, Shively, Christian A, Mitra, Robi D
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7229839/
https://ncbi.nlm.nih.gov/pubmed/32133534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa141
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