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Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications

The methanol utilization (Mut) phenotype in the yeast Pichia pastoris (syn. Komagataella spp.) is defined by the deletion of the genes AOX1 and AOX2. The Mut(−) phenotype cannot grow on methanol as a single carbon source. We assessed the Mut(−) phenotype for secreted recombinant protein production....

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Biotechnol Bioeng
Egile Nagusiak: Zavec, Domen, Gasser, Brigitte, Mattanovich, Diethard
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: John Wiley and Sons Inc. 2020
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7187134/
https://ncbi.nlm.nih.gov/pubmed/32034758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.27303
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