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Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications
The methanol utilization (Mut) phenotype in the yeast Pichia pastoris (syn. Komagataella spp.) is defined by the deletion of the genes AOX1 and AOX2. The Mut(−) phenotype cannot grow on methanol as a single carbon source. We assessed the Mut(−) phenotype for secreted recombinant protein production....
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| Veröffentlicht in: | Biotechnol Bioeng |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley and Sons Inc.
2020
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7187134/ https://ncbi.nlm.nih.gov/pubmed/32034758 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.27303 |
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