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Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris
BACKGROUND: The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to secretion. Simultaneous disruption of one vacuolar s...
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| Publié dans: | Microb Cell Fact |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6607557/ https://ncbi.nlm.nih.gov/pubmed/31269943 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1155-4 |
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