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Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

BACKGROUND: The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to secretion. Simultaneous disruption of one vacuolar s...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Marsalek, Lukas, Puxbaum, Verena, Buchetics, Markus, Mattanovich, Diethard, Gasser, Brigitte
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6607557/
https://ncbi.nlm.nih.gov/pubmed/31269943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1155-4
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