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Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

BACKGROUND: The yeast Pichia pastoris is a widely used host for the secretion of heterologous proteins. Despite being an efficient producer, we observed previously that certain recombinant proteins were mistargeted to the vacuole on their route to secretion. Simultaneous disruption of one vacuolar s...

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Bibliografische gegevens
Gepubliceerd in:Microb Cell Fact
Hoofdauteurs: Marsalek, Lukas, Puxbaum, Verena, Buchetics, Markus, Mattanovich, Diethard, Gasser, Brigitte
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BioMed Central 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6607557/
https://ncbi.nlm.nih.gov/pubmed/31269943
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-019-1155-4
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