Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

BACKGROUND: Inducible high-level expression is favoured for recombinant protein production in Pichia pastoris. Therefore, novel regulated promoters are desired, ideally repressing heterologous gene expression during initial growth and enabling it in the production phase. In a typical large scale fed...

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Autores principales: Prielhofer, Roland, Maurer, Michael, Klein, Joachim, Wenger, Jana, Kiziak, Christoph, Gasser, Brigitte, Mattanovich, Diethard
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2013
Materias:
Research
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3615954/
https://ncbi.nlm.nih.gov/pubmed/23347568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-12-5
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