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Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds

[Image: see text] For metabolic engineering approaches, fast and reliable tools are required to precisely manipulate the expression of target genes. dCas9 can be fused via RNA scaffolds to trans-activator domains and thus regulate the gene expression when targeted to the promoter region of a gene. I...

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Detalhes bibliográficos
Publicado no:ACS Synth Biol
Main Authors: Baumschabl, Michael, Prielhofer, Roland, Mattanovich, Diethard, Steiger, Matthias G.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2020
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7754189/
https://ncbi.nlm.nih.gov/pubmed/33180466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.0c00214
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