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Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability

Mammalian cytochrome P450 enzymes often metabolize many pharmaceuticals and other xenobiotics, a feature that is valuable in a biotechnology setting. However, extant P450 enzymes are typically relatively unstable, with T(50) values of ∼30–40 °C. Reconstructed ancestral cytochrome P450 enzymes tend t...

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Detalles Bibliográficos
Publicado en:J Biol Chem
Main Authors: Bart, Aaron G., Harris, Kurt L., Gillam, Elizabeth M. J., Scott, Emily E.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7186169/
https://ncbi.nlm.nih.gov/pubmed/32156703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA119.010727
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